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The same study found the binding affinity of MST/HN IgG1 Fc to have an approximate 35-fold lower affinity at pH 7.4 than at pH 6.0 (KD of ~33 nM)

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Even safety data on TB-500 (synthetic TB4) is relatively scarce, with only a few studies reporting the potential side effects of its systemic application: In a 2010 study in 10 participants, TB4 caused occasional mild to moderate symptoms like headache and dizziness but no serious reactions or toxicities, even at doses up to 1260mg, administered daily for up to two weeks

All available evidence is preclinical (rodent) only